Anti-LFA3/CD58 Antibody

Product Name

Anti-LFA3/CD58 Antibody

View all CD58/LFA-3 Antibodies

SKU/Catalog Number

RP1106

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LFA3/CD58 Antibody catalog # RP1106. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LFA3/CD58 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1106)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human LFA3 recombinant protein (Position: F29-R215).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1106 is reactive to CD58 in Human

Applications

RP1106 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

28.147kDa

Background of CD58/LFA-3

CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Flow Cytometry, 1-3μg/1x10⁶cells

Validation Images & Assay Conditions

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Western blot analysis of LFA3 expression in HELA whole cell lysates (lane 1) and K562 whole cell lysates (lane 2). LFA3 at 35KD was detected using rabbit anti-LFA3 Antigen Affinity purified polyclonal antibody (Catalog # RP1106) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

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LFA3 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti-LFA3 Antigen Affinity purified polyclonal antibody (Catalog # RP1106) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

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Figure 3. Flow Cytometry analysis of Hela cells using anti-LFA3 antibody (RP1106).
Overlay histogram showing Hela cells stained with RP1106 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LFA3 Antibody (RP1106,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CD58 (Source: Uniprot.org, NCBI)

Alternative Names

Ag3; CD58 antigen; CD58 antigen, (lymphocyte function-associated antigen 3); CD58 molecule; CD58; FLJ23181; FLJ43722; LFA3; LFA-3; LFA3ag3; lymphocyte function-associated antigen 3; Surface glycoprotein LFA-3 CD58 LFA-3, LFA3, ag3 CD58 molecule lymphocyte function-associated antigen 3|CD58 antigen, (lymphocyte function-associated antigen 3)|surface glycoprotein LFA-3

For more info on CD58, check out the CD58 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD58: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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